Lignocellulolytic activities among Trichoderma isolates from Lahad Datu, Sabah and Deception Island, Antarctic

Nur Shafawati Saili, and Shafiquzzaman Siddiquee, and Wong , Clemente Michael,Vui Ling and Marcelo González, and S Vijay Kumar, (2014) Lignocellulolytic activities among Trichoderma isolates from Lahad Datu, Sabah and Deception Island, Antarctic. Journal of Microbial and Biochemical Technology, 6 (5). pp. 295-302. ISSN 19485948

[img]
Preview
PDF
45Kb

Official URL: http://dx.doi.org/10.4172/1948-5948.1000159

Abstract

Trichoderma species have the potential for application in composting as biological control agents in controlling disease and increasing yield of production in the agricultural industry. The prevalent soil fungus of Trichoderma produces lignocellulolytic enzymes that assist the degradation of woody lignocellulose materials. The aim of the experimental work was to check the potential of lignocellulolytic Trichoderma fungi for the use of rapid composting of oil palm empty fruit bunches fibers. Fifty-two of Trichoderma isolates from Sabah and seven isolates from Antarctic were examined for in-vitro lignocellulolytic activity by assaying their ability to develop dark brown pigments, yellow halo zone, and clear white zone on tannic acid media (TAM) for lignin; Jensen Media (JM) for cellulose; and modified Melin-Nokrans media (MMNM) for starch. The best six Sabah Trichoderma isolates (5D, 10L2, 10P, 5E, 10X, and 10E2) were found to be potential lignocellulolytic agents based on their diameter of halo zone formed on amended media for further testing of in vitro bioconversion of oil palm empty fruit bunches. The diameters of halo zones were measured for the analysis of their ability in degrading lignin, cellulose, and starch. In contrast, Antarctic Trichoderma isolates consistently exhibited lower lignocellulolytic activities based on their smaller diameter of halo zone formed on TAM, JM, and MMNM. Most of the Trichoderma isolates are found to synthesize polyphenol oxidase, endoglucanases, and are able to hydrolyze starch to glucose in the three different media. Thus, the finding shows the potential of these isolates for use in large-scale composting of oil palm empty fruit bunches.

Item Type:Article
Uncontrolled Keywords:Antarctic; Genus Trichoderma; Lignocellulolytic; Oil palm empty fruit bunches
Subjects:Q Science > QH Natural history > QH301 Biology
ID Code:10149
Deposited By:IR Admin
Deposited On:12 Dec 2014 15:11
Last Modified:16 Feb 2015 15:10

Repository Staff Only: item control page


Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository