Physicochemical, phytochemical and antimicrobial properties of wild honey collected at mangrove and mountain areas in Sabah, Malaysian Borneo

Philip Yap , and Mohd Fadzelly Abu Bakar, (2014) Physicochemical, phytochemical and antimicrobial properties of wild honey collected at mangrove and mountain areas in Sabah, Malaysian Borneo. International Journal of Pharmacy and Pharmaceutical Sciences, 67 (3). pp. 437-447. ISSN 0975-1491

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Abstract

Objective: The aim of this study was to determine the physicochemical, phytochemical content and antimicrobial properties of selected honey of Sabah, Malaysian Borneo. Methods: A standardized protocols were used to evaluate the physicochemical properties of selected honey of Sabah while the phytochemicals content (phenolics and flavonoids) were determined using Folin-Ciocalteau and aluminium colorimetric methods. Antimicrobial properties were evaluated using disc diffusion assay. Results: For 80% methanol extract, old Upper Mountain honey contained the highest free acidity, conductivity, total phenolic and flavonoid contents with the values 23.84 ± 0.42 ml/g, 0.61 ± 0.01 mS/cm, 9.71 ± 0.01 mg gallic acid equivalent (GAE)/g and 7.76 ± 0.02 mg rutin equivalent (RU)/g, respectively. Antimicrobial activity showed strong inhibition by old Upper Mountain honey extract (80% methanol extract) with the value of 6.00 ± 0.01 mm at concentration of 100% against gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis). The same trend of phytochemicals content and antimicrobial activity was also observed in absolute methanol extracts. Conclusion: The present results suggested that wild raw honey collected at mangrove and mountain area in Sabah contained a wide range of phytochemical compounds which has the potential for human health.

Item Type:Article
Uncontrolled Keywords:Antimicrobials; Physicochemical; Phytochemicals; Wild honey
Subjects:R Medicine > RS Pharmacy and materia medica
ID Code:10383
Deposited By:IR Admin
Deposited On:28 Jan 2015 11:10
Last Modified:17 Feb 2015 11:29

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