Purification of chitinase produced by Streptomyces BRI 36

Selyvester @ Julkarnain Kundian, (2006) Purification of chitinase produced by Streptomyces BRI 36. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Spore suspension of Streptomyces BRI 36 was inoculated in 100 ml of enzyme production media in two batches. The synthesis of an extracellular Chitinase by Streptomyces BRI 36 was induced by using colloidal chitin as its sole carbon source. Chitinase was isolated from the culture filtrate and purified by acetone precipitation, Sephadex G-25, Sephadex G-50 and Sephadex 0-100 gel filtration. Enzyme assay was done by using colloidal chitin stained with Remazol Brilliant Blue® as substrate. Final yield was about 21 % for batch I and 9% for batch 2. Total protein was estimated by using modified Lowry method and given estimation of 5.15 mg for batch 1 and 1.88 mg for batch 2.

Item Type: Academic Exercise
Uncontrolled Keywords: Streptomyces BRI 36, enzyme, colloidal chitin, culture filtrate, protein
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 27 Jan 2015 05:52
Last Modified: 24 Oct 2017 05:51
URI: http://eprints.ums.edu.my/id/eprint/10406

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