Spectrophotometric determination of phosphate in water

Chay, Siew Leng (2006) Spectrophotometric determination of phosphate in water. Universiti Malaysia Sabah. (Unpublished)


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Spectrophotometric analysis of phosphate according to ascorbic acid method has been studied. Absorbance measurement of phosphate was carried out at λ= 880 nm using UV-VIS spectrophotometer. For low phosphate concentrations (<1 µg/mL), a maximum absorbance value was obtained within 10 minutes after mixing of reagent while at higher concentrations, a maximum was not attained within 60 minutes. The relationship between absorbance and concentration was in accordance to the Beer-Lambert law for the phosphate concentration range 0-1 µg/mL while higher concentrations resulted a negative deviation from the Beer's law. The presence of chloride, nitrate, sulphate, nitrite, Ca²� and Mg²� ions in solution did not interfere in the phosphate analysis. By contrast, the presence of arsenate, silicate and Cr�� ions interfered, particularly at higher concentrations

Item Type: Academic Exercise
Uncontrolled Keywords: Spectrophotometric, phosphate, ascorbic acid method, UV-VIS spectrophotometer, absorbance, Beer's law
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > School of Science and Technology
Depositing User: ADMIN ADMIN
Date Deposited: 06 Feb 2015 01:24
Last Modified: 24 Oct 2017 06:09
URI: http://eprints.ums.edu.my/id/eprint/10464

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