Membrane based detection of genetically modified organisms in some representatives food

Cheah, Yoke Kqueen and Radu, Son and Wong, Clemente Michael Vui Ling (2006) Membrane based detection of genetically modified organisms in some representatives food. Food Control, 17 (8). pp. 631-636. ISSN 0956-7135

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Abstract

Recently, DNA-based techniques became very common for the detection of genetically modified organisms (GMOs) in food products. For rapid and easy detection of GMOs, polymerase chain reaction (PCR) screening methods.. which amplify common transgenic elements, are applied in routine analysis. Incorporation of PCR and membrane method introduced in this study offer an alternative detection of GMOs. I it this study, a total of 32 samples and three certified reference materials were tested for the existence of the 35S promoter of cauliflower mosaic virus (CaMV) and 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene residues. Dot blot screening system introduced in this study can be routinely used as a semi-quantitative screening of GMOs. (c) 2005 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Cauliflower mosaic virus, 35S promoter, Genetically modified organisms, Polymerase chain reaction, Dot blot
Subjects: T Technology > TX Home economics > Nutrition. Foods and food supply
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 03 Oct 2011 10:05
Last Modified: 19 Oct 2017 01:32
URI: http://eprints.ums.edu.my/id/eprint/1111

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