Potentiality of locally isolated purple non-sulfur bacteria as aquaculture feed supplement in finfish larviculture system

Sankari Ranjapan, (2012) Potentiality of locally isolated purple non-sulfur bacteria as aquaculture feed supplement in finfish larviculture system. Masters thesis, Universiti Malaysia Sabah.

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Abstract

Six strains of purple non sulfur bacteria (UMSSTR1, UMSSTR2, UMSSTR3, UMSSTR4, UMSSTR5 and UMSSTR6) were isolated from the different locations of UMS beach sediments with an aim to evaluate the potentiality as an aquaculture feed supplement They were grown In 112 synthetic media under anaerobic condition at 2500 lux light illumination at 30±2°C temperature. Initial screening was done based on their growth characteristics in terms of dry cell biomass (g/L), production of total carotenoids (mg/g dry cell weight) and content of bacteriochlorophyll. The highest total carotenoid of 3.53±0.46 mg/g dry cell weight was obtained at 24hr culture in the strain UMSSTR1, but the highest dry cell weight of 5.67±0.42 g/L was obtained at 48hr culture in the strain UMSSTR2. No significant differences were observed in dry cell weight and the production of total carotenoid (p>0.05) among the six strains. Two strains (UMSSTR1 and UMSSTR2) were selected for identification based on 16S rRNA. They were identified as Rhodovulvum sulphidophilum (Rhv. sulphidophilum). Mass cultivation of the highest produced biomass strain UMSSTR2, Rhv. sulphidophilum was selected for finfish feeding trials. Two feeding trials were conducted to observe the growth and survival . of finfish larvae with traditional live feed Incorporated with Rhv. sulphidophilum bacteria biomass. The first feeding trial was done in African catfish (Clarias gariepinus) larvae and the second feeding experiment was conducted In River catfish (Pangasius hypophthalmus) larvae. Six experimental feeds diets were prepared and used in both feeding trials. The experimental diets were: the fresh bacterial biomass of Rhv. sulphidophilum as diet 1, Nannochloropsis as diet 2, Memia nauplii as diet 3, Rhv. sulphidophilum +Nannochloropsis as diet 4, Rhv. sulphidophilum + Memia as diet 5 and Rhv. sulphidophilum +Artemia+Nannochloropsis as diet 6. In feeding trial with C gariepinus larvae, the highest growth in length of 2.58±0.65cm, 23.15% specific growth rate and the highest survival of 90±4% obtained with diet 6. The results shows no significant differences (p>0.05) in the length and survival of the C. gariepinus larvae among six types of diets. The second feeding experiment was conducted with P. hypophtalmus larvae for the period of 20 days. The highest growth of 2.73±0.2cm with 11.55% specific growth rate and the survival of 86±2% were observed with larvae fed with diet 6. Significant differences (p<0.05) were observed in length and survival of P. hypophtillmus larvae fed with six types of diets. Both the experiments showed increment in the growth and survival in larvae. The bacterium strain UMSSTR2, Rhv. sulphidophllum Incorporated with the Artemia . and Nannochloropsis act as feed supplement for larval diet and enhanced the growth and survival in C. gariepinus and P. hypophtalmus larvae. Thus, the locally isolated bacterium Rhv. sulphidophilum has the potentiality to be used as aquaculture feed additive in addition to other live feed during larval rearing period of C gariepinus and P. hypophtalmus in larviculture system

Item Type:Thesis (Masters)
Uncontrolled Keywords:non sulfur bacteria, feed supplement, anaerobic, synthetic media, dry cell biomass, finfish
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:SCHOOL > Borneo Marine Research Institute
ID Code:11208
Deposited By:IR Admin
Deposited On:16 Jun 2015 15:47
Last Modified:16 Jun 2015 15:47

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