Experimental infection of Vibrio harveyi to seabass (Lates calcarifer) juvenile under different salinity

Nik Siti Zaimah Safiin, (2010) Experimental infection of Vibrio harveyi to seabass (Lates calcarifer) juvenile under different salinity. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Experimental fish, Seabass (Lates calcarifer) is an euryhaline species that can tolerate to wide range salinity. An experimental infection was conducted to examine the pathogenicity of Vibrio harveyi to seabass juvenile under different salinity level. There were five treatments which were 10, 15, 20, 25 and 30 ppt. Each of the salinity level was set as the control. Every salinity level of the treatment tank was done triplicate. The observation period were 10 days. Every tank was stocked with 6 individuals of seabass. V.harveyi was immersed into the treatment tanks while all of the control tanks were without Immersion. Within 10 days-observation, external clinical signs were observed. The treatment water were sampled every three days to identify the bacterial cell density in water by calculating colony forming unit. The moribund fish were dissected to Identify the internal signs of Infection on viscera organs. The viscera organ were homogenized for DNA extraction and spread on Vibrio Agar (VA) to calculate the bacterial cell density in tissue sample. By using MYVHEasy Detection Kit, Polymerase Chain Reaction (PCR) was run to detect the presence of pathogenic V.harveyi bands. The datas were analysed by using SPSS-One Way ANOVA. The results showed that the lowest survival rate and highest bacterial density in tissue and water as in 30 ppt. While, the highest survival rate and lowest bacterial density in tissue and water as in 10 ppt Analyses of data revealed that there were significant difference among six treatments (P<0.05) on survival, bacterial growth in water and bacterial density in tissue sample. All the treatment samples showed that the presence of pathogenic V.Harveyi while the control showed the absence of V.harveyi. Histology result showed the section of kidney with acute vibriosis of infected seabass with necrosis and haemorrhage while non-Infection occur on control fish.

Item Type:Academic Exercise
Uncontrolled Keywords:Lates calcarifer, experimental infection, Vibrio harveyi, salinity
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:SCHOOL > School of Science and Technology
ID Code:11729
Deposited By:IR Admin
Deposited On:09 Nov 2015 14:16
Last Modified:09 Nov 2015 14:16

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