Hydrolysis of DNA by a dipeptides containing histidine

Fan, Hui Yin and Zhao, Yu-fen and Ye, Yong and Liu, Cun-jiang and Guo, Bao-bao and Wang, Xu-bin and Zhu, Xiao-fei and Fan, Min (2010) Hydrolysis of DNA by a dipeptides containing histidine. International Journal of Peptide Research and Therapeutics, 16 (4). pp. 297-300. ISSN 1573-3904


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Three ligands which contain histidine and conjugated by a flexible linker, have been characterized and evaluated as DNA cleavage agents. The cleavage activity of metal complexes were evaluated by monitoring the conversion of supercoiled plasmid DNA (pUC19) (Form I) to nicked circular DNA (Form II) by agarose gel electrophoresis. The results showed that the cleavage activity of Cu(II) complexes was enhanced compared with histidine. Specially, at a high reaction concentration (0.2 mM), Cu(II) complexes can cleave the plasmid DNA with some selectivity.

Item Type: Article
Uncontrolled Keywords: DNA cleavage, Artificial nuclease, Cu(II) complexes
Subjects: T Technology > TP Chemical technology
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: ADMIN ADMIN
Date Deposited: 07 Jan 2016 05:45
Last Modified: 13 Oct 2017 01:48
URI: http://eprints.ums.edu.my/id/eprint/12458

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