Purification and some properties of Aspergillus pulverulentus β-xylosidase with transxylosylation capacity

Joko Sulistyo, and Yoshi Kamiyama, and Tsuneo Yasui, (1995) Purification and some properties of Aspergillus pulverulentus β-xylosidase with transxylosylation capacity. Journal of Fermentation and Bioengineering, 79 (1). pp. 17-22. ISSN 1389-1723

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Abstract

Two β-xylosidases [EC 3.2.1.37], β-Xyl I (molecular mass 180 kDa, pI 4.7) and β-Xyl II (molecular mass 190 kDa, pI 3.5), derived from Aspergillus pulverulentus were separated and purified by successive chromatographies and their characterization and transxylosylation were studied. β-Xyl I and β-Xyl II were stable at temperatures up to 50°C and from pH 1.5 to 6.5 and 2.5 to 7.0, respectively, while their highest activities were in the pH ranges 2.5–3.5 and 4.0–5.0 at 60°C. Although both enzymes were strongly inhibited by N-bromosuccinimide, the inhibitory effect of HgCl2 was not significant on either. The two enzymes exhibited different resistances against AgNO3, glucono 1,5-lactone and nojirimycin. They were shown to have broad acceptor specificity in transferring the xylosyl residue of xylooligosaccharides to various alcohol and phenolic compound acceptors. In the presence of 25% or more 2-propanol, the synthesis of the transfer product, 2-propyl β-xyloside, was closely consistent with the theoretical yield.

Item Type: Article
Uncontrolled Keywords: β-xylosidase, Aspergillus pulverulentus, transxylosylation
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 11 May 2016 06:06
Last Modified: 13 Oct 2017 06:35
URI: http://eprints.ums.edu.my/id/eprint/12622

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