Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials

Mailin Misson, and Firdausi Razali, (2007) Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials. Jurnal Teknologi, 46 (F). pp. 51-59. ISSN 2180-3722

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Abstract

The performance of two types of inert support, namely bioceramic and sponge to immobilize a locally isolated phenol degrader Pseudomonas sp. in a packed column was investigated in repeated batch culture. Prior to this, our study indicated that immobilization had doubled the tolerance limit of the cells towards phenol from 1000 ppm (in the suspended culture), to 2000 ppm. For the same volume, the bioceramic managed to trap bacterial cells 1.8 times greater than the sponge did. As a result, it was able to remove 100% of 1000 ppm 600–ml phenol fed at a rate of 2.5 ml/min within 24 hours, and the phenol removal capacity was sustained in the next six consecutive batches. Cells entrapped in sponge however, managed to remove around 90% phenol in five batches. Despite lower performance, at large scales, the use of sponge for cell entrapment offers some merits such as lightness, and easily available at cheaper cost.

Item Type: Article
Uncontrolled Keywords: Immobilization, phenol, Pseudomonas sp, bioceramic, repeated batch
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 07 Mar 2016 06:15
Last Modified: 16 Oct 2017 07:21
URI: http://eprints.ums.edu.my/id/eprint/12807

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