Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation

Clemente Michael Vui Ling Wong, and Mookiah Saminathan, and Chin, Chin Sieo and Norhani Abdullah, and Yin, Wan Ho (2015) Effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on in vitro methane production and rumen fermentation. Journal of the Science of Food and Agriculture, 95 (13). pp. 2742-2749. ISSN 1097-0010

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Abstract

BACKGROUND Molecular weights (MWs) and their chemical structures are the primary factors determining the influence of condensed tannins (CTs) on animal nutrition and methane (CH4) production in ruminants. In this study the MWs of five CT fractions from Leucaena leucocephalahybrid-Rendang (LLR) were determined and the CT fractions were investigated for their effects on CH4 production and rumen fermentation. RESULTS The number-average molecular weight (Mn) of fraction F1 (1265.8 Da), which was eluted first, was the highest, followed by those of fractions F2 (1028.6 Da), F3 (652.2 Da), F4 (562.2 Da) and F5 (469.6 Da). The total gas (mL g−1 dry matter (DM)) and CH4 production decreased significantly (P < 0.05) with increasing MWs of the CT fractions, but there were no significant (P > 0.05) differences between the CT fractions and control on DM degradation. However, the in vitro N disappearance decreased significantly (P < 0.05) with the inclusion of CT fraction F1 (highest MW) compared with the control and other fractions (F2–F5). The inclusion of CT fraction F1 also significantly decreased (P < 0.05) total volatile fatty acid and acetic acid concentrations compared with the control. The acetic/propionic acid ratio was significantly decreased (P < 0.05) by fraction F1 but not by the control and other fractions (F2–F5). CONCLUSION The CT fractions of different MWs from LLR could affect rumen fermentation and CH4 production, and the impact was more pronounced for the CT fraction with a higher MW.

Item Type: Article
Uncontrolled Keywords: condensed tannin fractions, molecular weight, Leucaena leucocephala hybrid-Rendang, methane, volatile fatty acids
Subjects: S Agriculture > S Agriculture (General)
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 24 Feb 2016 02:43
Last Modified: 11 Oct 2017 06:56
URI: http://eprints.ums.edu.my/id/eprint/12868

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