Polymorphism of cytochrome P450 in gastrointestinal cancer

Tung, En En (2008) Polymorphism of cytochrome P450 in gastrointestinal cancer. Universiti Malaysia Sabah. (Unpublished)


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The cytochrome P450 2E1 (CYP2E1) is an enzyme involved in the metabolism of carcinogenic chemicals. The polymorphism of the gene will result in changes of the enzyme chemical metabolism activities and lead to various cancers. The objective of this study is to investigate the association between RsaI polymorphism of CYP2E1 and risk of gastrointestinal cancer. A total of 11 gastrointestinal cancer cases and one control case were examined. The polymorphism was detected through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. This technique involves DNA amplification through PCR and followed by RsaI restriction enzyme digestion. Analysis of the digested products was performed on agarose gel electrophoresis to determine the genotypes. Next, statistical analysis was carried out to determine the association of the polymorphism towards gastrointestinal cancer. In this study, three different patterns of polymorphism with homozygous wildtype c1c2, heterozygous c1c2 and homozygous variant c2c2, were detected among the samples. The allele frequency of wildtype c1 allele was higher in gastrointestinal cancer cases (0.7) while the frequency of variant c2 allele was higher in control cases (0.5). The highest genotype frequency was found in the homozygous wildtype c1c1 in gastrointestinal cancer cases. The odd ratio between the two cases was lower than one (0.429). This value indicates that there is no association between the RsaI polymorphism and risk of gastrointestinal cancer. However, the finding was not significant due to the small sample size. Hence, further analysis with larger sample size should be carried out to obtain a more significant result.

Item Type: Academic Exercise
Uncontrolled Keywords: enzyme, carcinogenic chemicals, gastrointestinal cancer, RsaI polymorphism, cytochrome P450 2El
Subjects: R Medicine > RB Pathology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 02 Jun 2016 01:53
Last Modified: 30 Oct 2017 03:12
URI: http://eprints.ums.edu.my/id/eprint/13221

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