Genomic DNA fingerprinting of grouper from Sabah, Malaysia

Kah, Oi Wah (2012) Genomic DNA fingerprinting of grouper from Sabah, Malaysia. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Genetic relatedness between species can be applied to determine the viability of intraspecific hybrids. A study was carried out to determine the genetic relatedness between Epinephelus lanceolatus, E. fuseoguttatus, E. coioides and E. corallicola using randomly selected single locus DNA markers. A total of 27 DNA markers from E. lanceolatus were able to cross-amplify 15 and 7 loci in E. corallicola and E. coioides respectively indicating that E. lanceolatus is genetically related to E. corallicola. On the other hand 27 markers derived from E. fuscoguttatus failed to amplify in E. corallicola and only one locus amplified in E. coioides. This provides a strong indication of the fact that E. fuscoguttatus does not share a genetic similarity with these two species. These results of this study provide a basis for developing F1 hybrids from E. fuscoguttatus and E. corallicola as well as E. coioides due to their large genetic distance.

Item Type: Academic Exercise
Uncontrolled Keywords: single locus DNA markers, genetic, hybrid, E. corallicola, Epinephelus lanceolatus, E. fuseoguttatus, E. coioides
Subjects: Q Science > QH Natural history > QH426 Genetics
Divisions: SCHOOL > School of Science and Technology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 02 Jun 2016 01:53
Last Modified: 30 Oct 2017 03:20
URI: http://eprints.ums.edu.my/id/eprint/13297

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