Total polyphenol content, total flavonoid content and antioxidant activity of etlingera elatior flower

Siti Ayu Aziz, (2014) Total polyphenol content, total flavonoid content and antioxidant activity of etlingera elatior flower. Universiti Malaysia Sabah. (Unpublished)

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Abstract

In this study, the total polyphenol, flavonoid and antioxidant activity of essential oil of Etlingera elatiorwere investigated. The essential oil of E. elatior flower was extracted by using hydrodistillation and the chemical composition were analysed by using GCMS. The major component of the essential oil of E. elatior flower were 1-dodecanol (60.80 %), dodecanal, and 1,1-dodecanediol, diacetate with 32.27 %, methylcyclopentane (16.39 %), heptane (5.92 %), lauryl acetate (5.91 %), cyclotetradecane (5.05%) and a-pinene (2.32 %) with the percentage yield of essential oil of 0.031 %. The total polyphenol were determined by using Folin-Ciocaltue method and external calibration with Gallic acid while the total flavonoid content were determined by using aluminium chloride colorimetric method. The antioxidant activity of essential oil of E. elatior flower were measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay by measured its percentage of inhibition activity reduced by 50 % of DPPH (IC₅₀ %).The total polyphenol content of essential oil of E. elatior flower is 73.1± 0.0001 mg GAE/g while the total flavonoid content is 190.200 ± 0.0003 mg QE/g. The antioxidant activity of essential oil of E. elatior flower is low than synthetic antioxidant of butylated hydroxytouluene (BHT) with the value of 0.03 mg/mL of IC₅₀, while the IC₅₀ value of BHT is 0.00446 mg/mL. Thus it is clearly shows that the antioxidant activity of essential oil of E. elatior flower Is low, since the higher the antioxidant activity, the lower the value of IC₅₀.

Item Type: Academic Exercise
Uncontrolled Keywords: essential oil, Etlingera elatior, flavonoid, antioxidant activity, total polyphenol, hydrodistillation
Subjects: Q Science > QK Botany
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 28 Jun 2016 05:27
Last Modified: 30 Oct 2017 03:36
URI: http://eprints.ums.edu.my/id/eprint/13318

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