Isolation and characterization of antimicrobial proteins from fruits (kiwi, banana and coconut)

Nur Ramziahrazanah Jumat, (2010) Isolation and characterization of antimicrobial proteins from fruits (kiwi, banana and coconut). Universiti Malaysia Sabah. (Unpublished)

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Abstract

Antimicrobial proteins can be found In organisms ranging from bacteria to plants and mammals. Researches have been focusing on studying the antimicrobial proteins as infections caused by pathogenic bacteria and fungi can cause an expressive negative impact on both human and plants. Based on this study, antimicrobial proteins were extracted from the kiwi fruit, banana and coconut via three different methods of extraction of sodium chloride extraction, Tris-HCI buffer extraction and acetic acid extraction. Bradford assay was then carried followed by screening for antimicrobial activity and concentration dependency test. Lastly, the 50S-PAGE was carried out and the crude protein samples were purified via gel filtration chromatography. The highest crude protein recovery was achieved through the extraction of the antimicrobial proteins via Tris-HCI buffer extraction. The growth of both gram positive bacteria and gram-negative bacteria of 8. cereus,s. aureus, S. thyphimurium and E. Coli were able to be inhibited by antimicrobial proteins extracted from kiwi via Tris-HCI buffer and acetic acid, and also banana and coconut via sodium chloride. However, the antimicrobial proteins extracted from the fruits samples do not show any inhibition activity towards B. cinerea. Protein bands for M.a.1, C.n.1, A.d.2,M.a.2, C.n.2 and A.d.3 extracted via sodium chloride, Tris-HCI buffer and acetic could dearly be observed and has the size within the range of 14.4 kDa- 20 kOa,24 kOa-30 kDa and 30- kDa-43 kDa. The protein bands for the other fruits samples were not clearly visualized on the SDS-PAGE gel. The crude antimicrobial proteins were then purified using gel filtration chromatography which resulted in high protein recovery of the purified proteins for the fruit samples of kiwi, banana and coconut which corresponds to the high absorbance peaks which were obtained at 280 nm. Further studies on antimicrobial proteins extracted from fruits should be carried out to help in the treatment of several pathogen-infected diseases.

Item Type: Academic Exercise
Uncontrolled Keywords: antimicrobial, proteins, kiwi fruit, extraction, sodium chloride, Tris-HCI buffer, acetic acid
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > School of Science and Technology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 28 Jun 2016 03:45
Last Modified: 07 Nov 2017 03:09
URI: http://eprints.ums.edu.my/id/eprint/13533

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