Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning

Suryani Saallah, and M. Nazli Naim, and I. Wuled Lenggoro, and Mohd Noriznan Mokhtar, and Noor Fitrah Abu Bakar, and Masao Gen, (2016) Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning. Biotechnology Reports, 10. pp. 44-48. ISSN 2215017X

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Abstract

Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.

Item Type: Article
Uncontrolled Keywords: CGTase; Cyclodextrins; Electrospinning; Immobilisation; Nanofibres
Subjects: Q Science > QD Chemistry
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 13 Jul 2016 03:16
Last Modified: 23 Oct 2017 04:42
URI: http://eprints.ums.edu.my/id/eprint/13567

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