Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning

Suryani Saallah , and M. Nazli Naim , and I. Wuled Lenggoro, and Mohd Noriznan Mokhtar, and Noor Fitrah Abu Bakar , and Masao Gen, (2016) Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning. Biotechnology Reports, 10 . pp. 44-48. ISSN 2215017X

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Official URL: Http://dx.doi.org/10.1016/j.btre.2016.03.003

Abstract

Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (-9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film.

Item Type:Article
Uncontrolled Keywords:CGTase; Cyclodextrins; Electrospinning; Immobilisation; Nanofibres
Subjects:Q Science > QD Chemistry
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:13567
Deposited By:IR Admin
Deposited On:13 Jul 2016 11:16
Last Modified:13 Jul 2016 11:16

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