Screening for inhibitors of Protein Phosphatase (PP1), Mapk Kinase(MKK1) and map kinase phosphatase (MSGS) using actinomycete strains involved in eukaryotic signal transduction

Muhammad Naim Razali, (2010) Screening for inhibitors of Protein Phosphatase (PP1), Mapk Kinase(MKK1) and map kinase phosphatase (MSGS) using actinomycete strains involved in eukaryotic signal transduction. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The aim of this study is to search for potential inhibitor of Protein phosphatase-1 (PP1), Mitogen activated protein kinase kinase (MKK1), and also Mitogen activated protein kinase phosphatase (MSG5) from actinomycete strains that involved in eukaryotic signal transduction using yeast as a eukaryotic model. For PP1 screening, two types of yeast strains used are the mutant-type PAY 700-4 and the wild-type PAY 704-1. Mutant-type yeast carries the mutant-type gene of GLC7-10 while the wildtype yeast carries the wild-type GLC7 gene. For MKK1 screening, the mutant type yeast strain used was MKK1P386. MKK1P386 gene which carried by the yeast was induced by the GAL1promoter in response of glucose limitation. While the mutant type yeast strain, MKK1P386-MSGS, was used in the screening of MSG5 inhibitors. The yeast carries the MKK1 P386-MSGS gene that will suppress the growth inhibitory effects of over expression of MKK1 P386 gene. There are 40 strains of actinomycete are used. They are H1001, H1006, H1036, H1045, H1032, H1002, H1004, H1017, H7323, H7434, H7456, H7478, H7998, H8565, H8598, H8601, H8632, H8637, H8641, H8648, H8651, H8654, H8667, H8688, H8691, H8693, H8698, H8700, H8724, H8961, H8970, H8991, H11448, H11464, H11526, Hl1661, H11670, H11783, H11821, and H11841. These strains of actinomycete were taken from soil in several Sabah regions. The secondary metabolites from actinomycete strains were extracted using equal volume of acetone and screened for PPI, MKKI and MSGS Inhibitors. In each screening methods, 20µ of acetone extract with the concentration of 50mg/ml were placed to each paper disks. Distilled water was used as a negative control. Acetone extracts of each actinomycetes strain do not show any activity when screening against WI, MKK1, and MSG5 inhibitions and each of them also are n0n toxic. However, the possibility of potential inhibitors from actinomycete strains could not be excluded. Therefore, further investigation need to be done.

Item Type: Academic Exercise
Uncontrolled Keywords: Mutant-type yeast, inhibitor, Protein phosphatase-1, actinomycete strains, eukaryotic model
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > School of Science and Technology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 14 Jul 2016 02:09
Last Modified: 20 Nov 2017 03:34
URI: http://eprints.ums.edu.my/id/eprint/13598

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