Diagnosis of Genus Helicobacter through a hemi-nested PCR assay of 16S rRNA

Muhammad Aqeel Ashraf , and Qin , Heping and Tang , Guido and Yi , Ping and Pan , Xinyi and Huang , Huali and Chang , Renjie and Shi , Zhe (2016) Diagnosis of Genus Helicobacter through a hemi-nested PCR assay of 16S rRNA. Saudi Pharmaceutical Journal , 24 (3). pp. 265-272. ISSN 1319-0164

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Official URL: http://dx.doi.org/10.1016/j.jsps.2016.04.015

Abstract

The present study aimed to establish a genus-specific PCR-based assay to detect helicobacters using 16S rRNA gene as the target template. We designed the hemi-nested primers based on sequences of 16S rRNA gene of 34 types of Helicobacter species. The inclusivity, sensitivity, and specificity of the PCR assay using these primers were examined in three different models, comprising feces simulated samples, BLAB/c mice infection model and clinic patients samples. The detection sensitivity of Helicobacter pylori, Helicobacter hepaticus and Helicobacter bilis strains from feces simulated samples was all 102 CFU/ml. We successfully detected H. hepaticus and H. bilis in the liver, cecum and feces of experimentally infected mice. H. pylori was successfully detected in the feces samples from 3 patients infected with H. pylori while not in the feces samples from 3 healthy human. However, the C97/C05–C97/C98 PCR assay detected H. pylori in the 2 positive samples. Due to the PCR assay’s excellent inclusivity, high sensitivity and specificity it may be used to detect the presence of Helicobacters.

Item Type:Article
Uncontrolled Keywords:Helicobacter, Hemi-nested PCR assay, 16S rRNA
Subjects:R Medicine > R Medicine (General)
Divisions:FACULTY > Faculty of Science and Natural Resources
ID Code:13637
Deposited By:IR Admin
Deposited On:03 Aug 2016 14:19
Last Modified:03 Aug 2016 14:19

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