In-vitro evaluation of anti-kinase, anti-phosphatase and cytotoxic activities of Mikania micrantha H.B.K. (asteraceae) from Malaysia

Azlinah Matawali, and Lee , ping Chin and How , Siew Eng and Boon , Lee Hong and Jualang Azlan Gansau, (2016) In-vitro evaluation of anti-kinase, anti-phosphatase and cytotoxic activities of Mikania micrantha H.B.K. (asteraceae) from Malaysia. Journal of Chemical and Pharmaceutical Sciences, 9 (2). pp. 696-701. ISSN 9742115

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Abstract

Mikania micrantha H.B.K (Asteraceae) is a creepy with soft stem weed which also known locally as Selaput Tunggul. Although being considered among worst Invasive Alien Weed (IAW) species in the world with less biologically importance, it still has patronage from traditional practitioners as the remedy to cure insects or snake bite. This study reports other promising medicinal properties of this plant species. Dried leaves were extracted with various solvent systems, concentrated under reduced pressure and later evaluated for its anti-kinase, anti-phosphatase and cytotoxic activities. Both anti-kinase and anti-phosphatase assays targeted protein MKK1, MSG5 and PP1 in mutated yeast strains namely as MKK1P386, MKK1P386-MSG5, PAY704-1 and PAY700-4, respectively. The crude methanolic extract has observed as the only inhibitor for PP1 screening assay. Liquid-liquid partition of this extract has confirmed the chloroform partition exhibited potential activity against PP1. Further separation of this partition extracts using column chromatography yielded 5 fractions namely as F1 to F5. Fraction F2 was later confirmed as the PP1 inhibitor, while fraction F1 observed as toxic. MTT assay of this plant extracts also showed good cytotoxic activity against HL60 cell line. This result has indicated that M. micrantha shows promise as the natural anticancer agent.

Item Type:Article
Uncontrolled Keywords:Anti-cancer; HL-60; Malaysia; Mikania micrantha; Signal transduction
Subjects:Q Science > Q Science (General)
Divisions:FACULTY > Faculty of Science and Natural Resources
ID Code:13747
Deposited By:IR Admin
Deposited On:03 Aug 2016 14:12
Last Modified:03 Aug 2016 14:12

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