Development and application of single locus genomic molecular marker for Kappaphycus and Eucheuma (Solieriaceae, Rhodophyta) seaweeds

Kenneth, Francis Rodrigues and Vun , Yee Thien and Thau, Wilson Lym Yong and Ann Anton, and Wei, Grace Joy Lie Chin (2016) Development and application of single locus genomic molecular marker for Kappaphycus and Eucheuma (Solieriaceae, Rhodophyta) seaweeds. Aquatic Botany, 128 . pp. 26-32. ISSN 0304-3770

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Official URL: http://dx.doi.org/10.1016/j.aquabot.2015.09.006

Abstract

The division Rhodophyta includes members from the commercially important genera Kappaphycus and Eucheuma. The efficient cultivation of seaweeds depends upon the development of genomic molecular markers which can be applied to detect varietal differences and select strains which exhibit ideal growth characteristics. We developed 117 single loci DNA markers from shotgun genomic libraries derived from Kappaphycus alvarezii, Kappaphycus striatus and Eucheuma denticulatum and tested them across twelve phenotypes which are commercially cultured in Sabah, Malaysia. Sixteen of the markers amplified across all varieties of Kappaphycus implying a shared genetic identity. Eight of the markers could successfully differentiate between varieties of Eucheuma and Kappaphycus on the basis of DNA fingerprinting profiles. These markers will serve as resource for the identification and selection of red algae for commercial cultivation and their application can be extended to marker assisted selection (MAS) and hybrid development.

Item Type:Article
Uncontrolled Keywords:Seaweed, Small-insert genomic libraries, Single locus genomic molecular marker, DNA fingerprinting
Subjects:Q Science > Q Science (General)
Divisions:SCHOOL > Borneo Marine Research Institute
ID Code:13846
Deposited By:IR Admin
Deposited On:12 Aug 2016 11:32
Last Modified:12 Aug 2016 11:32

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