Cytotoxicity, cell cycle arrest, and apoptosis in breast cancer cell lines exposed to an extract of the seed kernel of Mangifera pajang (bambangan)

Mohd Fadzelly Abu Bakar, and Maryati Mohamed, Datin and Asmah Rahmat, and Burr, Steven A. and Fry, Jeffrey R. (2010) Cytotoxicity, cell cycle arrest, and apoptosis in breast cancer cell lines exposed to an extract of the seed kernel of Mangifera pajang (bambangan). Food and Chemical Toxicology, 48 (6). pp. 1688-1697. ISSN 0278-6915

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Official URL: http://dx.doi.org/10.1016/j.fct.2010.03.046

Abstract

An extract of Mangifera pajang kernel has been previously found to contain a high content of antioxidant phytochemicals. The present research was conducted to investigate the anticancer potential of this kernel extract. The results showed that the kernel crude extract induced cytotoxicity in MCF-7 (hormone-dependent breast cancer) cells and MDA-MB-231 (non-hormone dependent breast cancer) cells with IC50 values of 23 and 30.5 μg/ml, respectively. The kernel extract induced cell cycle arrest in MCF-7 cells at the sub-G1 (apoptosis) phase of the cell cycle in a time-dependent manner. For MDA-MB-231 cells, the kernel extract induced strong G2-M arrest in cell cycle progression at 24 h, resulting in substantial sub-G1 (apoptosis) arrest after 48 and 72 h of incubation. Staining with Annexin V-FITC and propidium iodide revealed that this apoptosis occurred early in both cell types, 36 h for MCF-7 cells and 24 h for MDA-MB-231cells, with 14.0% and 16.5% of the cells respectively undergoing apoptosis at these times. This apoptosis appeared to be dependent on caspase-2 and -3 in MCF-7 cells, and on caspase-2, -3 and -9 in MDA-MB-231 cells. These findings suggest that M. pajang kernel extract has potential as a potent cytotoxic agent against breast cancer cell lines.

Item Type:Article
Uncontrolled Keywords:Mangifera pajang; Cytotoxicity; Breast cancer; Apoptosis
Subjects:S Agriculture > S Agriculture (General)
Q Science > QK Botany
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions:SCHOOL > School of Medicine
SCHOOL > Institute for Tropical Biology and Conservation
ID Code:1453
Deposited By:IR Admin
Deposited On:27 Jan 2011 10:00
Last Modified:18 Feb 2015 09:55

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