Development of single locus molecular markers for epinephelus fuscoguttatus

Yuszniahyati Yaakob, (2012) Development of single locus molecular markers for epinephelus fuscoguttatus. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Epinephelus fuscoguttatus is a commercially important marine fish species in Southeast Asia and has been declared as near-threatened marine species by International Union for Conservation of Nature and Natural Resources. Based on single locus, molecular markers can be applied in the population studies as well as for the development of genomic databases which very useful in conservation programs. This project focused on the development of single locus molecular markers for Epinephelus fuscoguttatus. Epinephelus fuscoguttatus samples were obtained from Borneo Marine Research Centre of University Malaysia Sabah, Sabah. DNA from lateral fin was extracted by using modified salt extraction method. The extracted DNA was amplified by polymerase chain reaction (PCR) and the PCR products were ligated onto pUC19 cloning vector. This was followed by sequencing and development of six markers that can be used to amplified in the Epinephelus fuscoguttatus. A total of 20 sequences were isolated, 18 primers were designed and six primers amplified in the target species. These primers were used in the cross amplification process among the grouper species as well as the F1 hybrid grouper. These markers have a potential application in detection of the inheritance pattern among the grouper species as well as in the F1 grouper hybrids. Hence, through this application, these markers may be useful in the conservation and breeding programs of the grouper species.

Item Type:Academic Exercise
Uncontrolled Keywords:polymerase chain reaction (PCR), pUC19 cloning ,F1 hybrid grouper
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:FACULTY > Faculty of Science and Natural Resources
ID Code:14820
Deposited By:IR Admin
Deposited On:15 Nov 2016 14:57
Last Modified:15 Nov 2016 14:57

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