High integrity total RNA isolation from human peripheral blood that is as competitive to commercialize kits

Jia, Lim hong and Poh, Lucky, Wah Goh and Chong, Eric Tzyy Jiann and Lee, Ping Chin (2016) High integrity total RNA isolation from human peripheral blood that is as competitive to commercialize kits. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7 (4). pp. 1937-1942. ISSN 9758585

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Abstract

A substantial problem when isolating total RNA from human whole blood is the susceptibility to degradation and inconsistent yield of RNA especially when isolation is performed from clinical samples. Here, we report an improved method for isolating high integrity of total RNA from human peripheral blood using guanidium thiocyanate-phenol-chloroform. We found that this cost effective method was able to consistently produced high yield and high integrity of total RNA with average RNA concentration > 2000 ng/mL of blood and RNA integrity number (RIN) > 8.0, as well as negligible genomic DNA contamination from human whole blood that was comparable to other commercial blood total RNA isolation kits. In addition, the present method is also suitable for blood sample that required up to 24 hr transportation time prior total RNA extraction, and able to obtain consistent result in real-time polymerase chain reaction (qPCR) downstream application.

Item Type: Article
Uncontrolled Keywords: Human peripheral blood; QPCR; RNA integrity number; Total RNA isolation
Subjects: Q Science > QC Physics
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 16 Nov 2016 07:03
Last Modified: 23 Oct 2017 07:42
URI: http://eprints.ums.edu.my/id/eprint/14928

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