Ribosomal DNA analysis of marine microbes associated with toxin-producing Pyrodinium bahamense var compressum, a harmful algal bloom species

Teoh, Peik Lin and Kumaresan, Sivakumar and Ann Anton, and Chin, Grace Joy Wei Lie (2013) Ribosomal DNA analysis of marine microbes associated with toxin-producing Pyrodinium bahamense var compressum, a harmful algal bloom species. Pertanika Journal of Tropical Agricultural Science, 36 (2). pp. 179-188. ISSN 1511-3701

[img]
Preview
Text
Ribosomal_DNA_analysis_of_marine_microbes_associated_with_toxin.pdf

Download (49kB) | Preview

Abstract

Blooms of the toxic alga, Pyrodinium bahamense var. compressum (Böhm), have become a problem in Malaysia over the past three decades. The alga is a causative agent of paralytic shellfish poisoning, a potentially fatal neurological disorder. Past research suggest that bacteria-algae association may play a direct or indirect role in toxin production. As such, ribosomal DNA-based restriction enzyme analysis for the identification of bacteria associated with Pyrodinium sps. was undertaken. A total of 16 bacterial isolates were successfully obtained from the clonal cultures of Pyrodinium sps. The diversity of the extracellular bacteria associated with Pyrodinium bahamense var. compressum was limited to the Phyla Proteobacteria and Actinobacteria. The major bacterial species identified included Alcanivorax spp. and Hyphomonas spp., whereas Kocuria spp., Nesterenkonia spp., Alteromonas spp., Roseobacter spp., Xanthomonas spp., and Acinetobacter spp. were identified as minor isolates. The identified bacterium Hyphomonas spp. exhibited high sequence identity with an unknown bacterium strain, SCRIPPS 739, in the GenBank database that is known to be associated with toxic and non-toxic dinoflagellates, Alexandrium spp. and Scrippsiella trochoidea, respectively.

Item Type: Article
Uncontrolled Keywords: Bacteria, harmful algal bloom, paralytic shellfish poisoning, Pyrodinium bahamense var. compressum ribosomal DNA
Subjects: Q Science > QK Botany
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 19 Dec 2016 03:13
Last Modified: 25 Oct 2017 02:19
URI: http://eprints.ums.edu.my/id/eprint/15251

Actions (login required)

View Item View Item

Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository