Studies of sterilization protocol development and calli induction of selected tropical mosses

Makinde A.M., and Isa M.O, and Ayisire B.E., (2014) Studies of sterilization protocol development and calli induction of selected tropical mosses. Journal of Tropical Biology & Conservation (JTBC), 11. ISSN 1832-3902


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Four tropical African mosses namely; Racopilum africanum, Thuidium gratum, Archidium ohioense and Bryum coronatum, were studied. This was with a view to developing a sterilization protocol and inducing calli from the gametophyte explants in vitro. The mosses were collected from their natural populations in the Central Campus of the Obafemi Awolowo University, Ile-Ife, Nigeria. Healthy stem tips from the gametophytes of each of the mosses were sterilized with JIK (containing 3.85 % M/V NaOCI) and then cultured on Murashige and Skoog (MS) 1962 full strength medium, which served as the control and a full strength MS medium supplemented with 5 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D). Sterilization of the explants with 8 % JIK for 8 minutes was found to be most suitable. Only Bryum coronatum produced calli on hormone free medium, while the 2,4-D induced massive production of calli in Bryum coronatum and Racopilum africanum

Item Type: Article
Uncontrolled Keywords: Explant, Calli, Gametophyte, 2,4-D, Sterilization
Subjects: Q Science > QH Natural history
Depositing User: Users 83 not found.
Date Deposited: 10 Feb 2017 07:28
Last Modified: 11 Oct 2017 02:46

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