Effect of basal media and complex additives on seed germination of Coelogyne asperata (orchidaceae)

Arnnyitte Alexander, (2011) Effect of basal media and complex additives on seed germination of Coelogyne asperata (orchidaceae). Universiti Malaysia Sabah. (Unpublished)

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Abstract

The effects of different basal media and complex additives on in vitro seed germination of Coelogyne asperata were studied. Seeds of C. asperata after 182 days of pollination were cultured on three basal media of Knudson C (KC), Vacin and Went (VW), and Murashige and Skoog (MS) and maintained at 25±2°C under 24h light. After 40 days of culture (OAC), seeds were germinated on KC, MS and WI media at 96.45±2.68%, 95.51±3.01 % and 97.22±1.99% with growth Index (GI) valued 387.04, 375.24 and 379.79 respectively. The effect of complex additives was studied by using KC as basal medium, added with different types of complex additives such as 10% (v/v) of coconut water (CW), 10% (w/v) of potato homogenate (POT and 10% (w/v) of tomato juice (TJ). After 70 DAC, the germination percentage on KC (control), KC + 10% (v/v) CW, KC + 10% (w/v) POT and KC + 10% (w/v) TJ showed responsive germination percentage with GI value at 416.57, 375.4, 387.3 and 354.2 respectively. The finding concluded that C. asperata can be germinated better on basal media even without the supplementation of complex additives.

Item Type:Academic Exercise
Uncontrolled Keywords:basal media, in vitro, seed germination, coelogyne asperata
Subjects:S Agriculture > SB Plant culture
Divisions:FACULTY > Faculty of Sustainable Agriculture
ID Code:17011
Deposited By:Munira M
Deposited On:07 Jun 2017 14:29
Last Modified:07 Jun 2017 14:29

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