Effect of extraction solvents on total phenolic and total flavonoids contents and antimicrobial activities of Euphorbia hirta and Centranthera tranquebarica

Tan , Xue Yi (2014) Effect of extraction solvents on total phenolic and total flavonoids contents and antimicrobial activities of Euphorbia hirta and Centranthera tranquebarica. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The effect of two extracting solvents (ethanol and aqueous) on phytochemical constituents, total phenolic and flavonoids contents and antimicrobial activities of Euphorbia hirta and Centranthera tranquebarica were investigated in this study. Fresh samples were collected, dried, ground and extracted using soxhlet extractor prior to phytochemical and antimicrobial analyses. Data were analyzed using chi-square, independence T- test and two-way ANOVA at 5 % significant level and Duncan's test was performed for mean separation. The results showed that ethanolic plant extracts of E. hirta and C tranquebarica contained most of the phytochemical constituents such as flavonoids, glycosides, tannins, saponins, anthraquinones and diterpenes through preliminary screening test. The total phenolic contents of E. hirta and C tranquebarica was significantly higher in ethanolic extracts compared to the aqueous extracts with (0.2942 ± 0.002 mg GAE/g dry samples) and (0.2457 ± 0.002 mg GAE/g dry sample) respectively. Meanwhile, the total flavonoid contents of E. hirta and C tranquebarica was also higher in ethanolic extracts compared to the aqueous extracts with (0.1656 ± 0.002 mg QE/g dry sample) and (0.2473 ± 0.005 mg QE/g dry sample) respectively. For antimicrobial study, ethanolic extract of E. hirta showed the strongest inhibition against the growth of Escherichia coli (17.4 ± 2.302 mm), Pseudomonas aeroginosa (20.8 ± 0.837 mm), Bacillus cereus (15.8 ± 1.483 mm) and Staphylococcus aureus (16.2 ± 2.280 mm) compared to the aqueous extracts of the plant. Ethanolic extracts of C. tranquebarica also showed better inhibition towards the growth of P. aeroginosa (18.2 ± 1.304 mm), B. cereus (13 ± 2.000 mm) and S. aureus (12.4 ± 1.817 mm) compared to the aqueous plant extracts. Plant extracts with different quantity of secondary metabolites responsible for the broad spectrum of pharmacological activities towards antimicrobial activities. These results revealed that ethanol was the best extraction solvent which possesses more phytochemical constituents, total phenolic and flavonoids contents and antimicrobial activity against tested bacteria for E. hirta and C tranquebarica. Further research for different types of solvents extraction can be done in order to compare the quality and quantity of chemical constituents and antimicrobial activity for the selected plant species. Potential of antimicrobial effects against the bacteria show that the plant extracts can be used as a safe, reliable and economical natural antimicrobial source for the therapeutic reasons.

Item Type:Academic Exercise
Uncontrolled Keywords:soxhlet extractor, ethanol, aqueous, Euphorbia hirta, Centranthera tranquebarica
Subjects:S Agriculture > SB Plant culture
Divisions:FACULTY > Faculty of Sustainable Agriculture
ID Code:17035
Deposited By:Munira M
Deposited On:07 Jun 2017 14:31
Last Modified:07 Jun 2017 14:31

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