Effect of tetrazouum and conventional germination tests on five different short term high yielding rice varities

UNSPECIFIED (2015) Effect of tetrazouum and conventional germination tests on five different short term high yielding rice varities. Universiti Malaysia Sabah. (Unpublished)

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Abstract

This study was conducted in the net house of Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan Campus from April 2014 until October 2014 to determine the effect of tetrazolium and conventional germination tests on five short terms high yielding rice varieties. The objectives of this study were to determine the efficiency of both tetrazolium quick test and conventional germination test on the percentage/ degree of seed viability on different varieties of rice seeds and to compare the effidency between the seed tetrazolium quick test and conventional germination test on different varieties of rice seeds. The freshly harvested TQR-1, TQR-2, IR 72, TR 8 and TR 9 rice seeds were bisected and half of the seed was soaked in tetrazolium chloride for 2 to 4 hours and decanted before placed in an oven. The seeds also were germinated in germination boxes and were observed for 14 days. Completely Randomized Design was used in this experiment with three replicates for each test. Data for important parameters such percentage of germination and the number of viable seeds were analysed by two-way between group ANOVA while the parameters such as percentages of normal and abnormal seedlings, percentage of seedlings with plumule, percentage of seedlings with radicle, length of roots, height of seedlings and percentage of dead seeds for conventional germination test were compared with number of viable seeds. IR 72 (87.5%) resulted better in seed viability followed by TQR-2 (87%) when tested with tetrazolium quick test, while when tested with conventional germination test, TQR-2 (95.25%) resulted better in seed viability followed by TQR-1 (90.92%). The lowest mean seed viability for both tests was TR 8 (85.42% for tetrazolium and 72.84% for conventional germination tests). However, there were significant differences between both tests and rice varieties on Week 1 and 3 while on Week 5 and 7, there were no significant differences between both tests and rice varieties. Taking into consideration the percentages of seed viability tested with tetrazolium were compared to other parameters, it can be concluded that as seed viability increased, the percentage of normal and abnormal seedlings, height of seedlings, percentage of seedlings with plumule, percentage of seedlings with radicals also increased in all rice varieties tested but decreased in length of roots and the percentage of dead seeds. Therefore, combination with conventional test can be useful for testing dormant seed while the tetrazolium quick test gave the percentage of viable seed and the difference between both tests represent the percentage of dormant seeds. Besides, both tests can be used to determine the seed Viability. It is also be recommended use both tests in determining the seed viability which may give more accurate results and faster and also it is recommended to use tetrazolium test and followed or supported by conventional germination test in determining the seed viability due to some rice variety having seed dormancy.

Item Type:Academic Exercise
Uncontrolled Keywords:tetrazolium, germination tests, rice varieties, seed viability
Subjects:S Agriculture > SB Plant culture
Divisions:FACULTY > Faculty of Sustainable Agriculture
ID Code:17045
Deposited By:Munira M
Deposited On:08 Jun 2017 12:06
Last Modified:08 Jun 2017 12:06

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