Detection of water stress-induced gene in maize under drought stress condition

Wong, Jhonly Chin Hing (2011) Detection of water stress-induced gene in maize under drought stress condition. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Plant growth and productivity are greatly affected by water stress. Maize is an important cash crop in Malaysia where it often suffers from drought stress. This study focused on determines the water stress-induced gene in maize under drought stress condition in Sabah. The young maize leaves which at the weight range of 100 ± 5 mg were collected for total RNA Isolation process and the RNA yield range is 50-200 I-/g. There were undetectable amplified band in PCR process for Samples 1 and 2. While, a specific band located approximate 585 bp was amplified by using AHLEA primer set for Samples 3, 4 and 5. Sample 5 shown most clearance of amplified band which were proceed with purification process. This band was eluted and cloned into pGEM®-T Easy Vector. There were 10 white colonies and 11 light blue colonies were formed on LB plate supplemented with Ampicillin/ X-Gal/ IPTG. After miniprep isolation, these plasm ids were digested with EcoRI. Two different bands which located at 400 bp and 230 bp were formed by EcoRI. These result showed that there is stress-induced gene in maize under drought stress condition.

Item Type: Academic Exercise
Uncontrolled Keywords: maize, water stress-induced, RNA Isolation process
Subjects: S Agriculture > SB Plant culture
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Users 83 not found.
Date Deposited: 05 Jul 2017 01:56
Last Modified: 16 Oct 2017 04:00
URI: http://eprints.ums.edu.my/id/eprint/17360

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