Isolation and characterization of a molybdenum-reducing and phenolic and catechol-degrading enterobacter sp. strain Saw-2

Mohd Khalizan Sabullah, and Mohd Fadhil Rahman, and Siti Aqlima Ahmad, and Mohd Rosni Sulaiman, and Mohd Shukri Shukor, and Azlan Jualang Gansau, and Nor Aripin Shamaan, and Mohd Yunus Shukor, (2017) Isolation and characterization of a molybdenum-reducing and phenolic and catechol-degrading enterobacter sp. strain Saw-2. Biotropica, 24 (1). ISSN 1744-7429

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Abstract

Molybdenum is an emerging pollutant worldwide. The objective of this study is to isolate molybdenum-reducing bacterium with the ability to grow on phenolic compounds (phenol and catechol). The screening process was carried out on a microplate. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). The bacterium required a narrow pH range for optimal reduction of molybdenum, i.e. between pH 6.3 and 6.8, with temperature between 34 and 37 oC. Molybdate reduction to Mo-blue was best supported by glucose as the carbon source. However, both phenol and catechol could not support molybdate reduction. Other requirements for molybdate reduction included sodium molybdate concentrations between 15 and 30 mM, and phosphate concentration of 5.0 mM. The bacterium exhibited a Mo-blue absorption spectrum with a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. strain Saw-2. The capability of this bacterium to grow on toxic phenolic compounds and to detoxify molybdenum made it a significant agent for bioremediation.

Item Type: Article
Uncontrolled Keywords: Catechol, Enterobacter sp., Molybdenum, molybdenum blue, phenol
Subjects: Q Science > Q Science (General)
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 22 Mar 2018 02:06
Last Modified: 22 Mar 2018 02:06
URI: http://eprints.ums.edu.my/id/eprint/18620

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