A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity

Mohd Khalizan Sabullah, and Fisal Ahmad, and Azlan Jualang Gansau, and Mohd Rosni Sulaiman, (2015) A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity. Bioremediation Science and Technology Research, 3 (2). pp. 26-29. ISSN 2289-5892

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Abstract

Observations on the effects of copper on the liver proteome of Puntius javanicus based on the one dimensional PAGE was carried out. The liver was dissected from each fish, which was separately treated with different concentrations of copper sulfate ranging from 0.1 to 5.0 mg/L. The livers were extracted and one dimensional PAGE was performed under nonreducing (native) and reducing (SDS)-PAGE. Several bands were resolved in the native PAGE with probable candidates for the effect of copper observed showing an increased in the expression and down regulation strongly associated with increasing copper concentrations. This study showed that high concentrations of copper significantly alters P. Javanicus liver at the proteome level, and preliminary screening based on one dimensional PAGE is considered rapid and simple to assess the toxicity effect of copper before more advanced and extensive assessment with a second dimensional PAGE is carried out.

Item Type: Article
Uncontrolled Keywords: Puntius javanicus, 1-D PAGE, native PAGE, SDS-PAGE, copper
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 18 Feb 2018 11:56
Last Modified: 18 Feb 2018 11:56
URI: http://eprints.ums.edu.my/id/eprint/18786

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