Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation

Clarence M. Ongkudon, and Michael K. Danquah, (2014) Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation. Separation Science and Technology, 49 (1). pp. 39-46.

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Abstract

In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endotoxin assay. This work concludes that Zn2+ (as ZnSO4) optimally binds to and increases the hydrodynamic size of endotoxins compared to Mg2+ and Ca2+. A low polydispersity index value measured after cation addition indicates the presence of homogenously suspended supramolecular endotoxin particles which calls for a centrifugal separation step for complete endotoxin removal.

Item Type: Article
Uncontrolled Keywords: aggregation, divalent metal cation, endotoxin removal, Limulus Amoebocyte lysate, lipopolysaccharide
Subjects: Q Science > Q Science (General)
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Munira
Date Deposited: 18 Feb 2018 11:57
Last Modified: 18 Feb 2018 11:57
URI: http://eprints.ums.edu.my/id/eprint/18874

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