Comparision of staining methods for two dimensional electrophoresis gel resolved with Puntius javanicus liver proteome

Mohd Khalizan Sabullah, and Siti Aqlima Ahmad, and Fisal Ahmad, and Siti Aimi Sarah Zainal Abidin, and Azlan Jualang Gansau, and Mohd Rosni Sulaiman, (2014) Comparision of staining methods for two dimensional electrophoresis gel resolved with Puntius javanicus liver proteome. Journal of Biochemistry, Microbiology and Biotechnology, 2 (1). pp. 27-31. ISSN 2289-5779

[img]
Preview
Text
Comparision of staining methods for two dimensional electrophoresis gel resolved with Puntius javanicus liver proteome.pdf

Download (47kB) | Preview

Abstract

The aim of this study was to compare the various staining methods based on commassie briliant blue and silver nitrate stain for the two dimensional gel electrophoresis resolved with Puntius javanicus liver proteome. The staining methods were selected base on the previous report about their compatibility with the mass spectrometry analysis. Silver staining method is known as the most sensitive method to visualize the maximum number of protein spots resolved in 2D gel but it is less sensitive(incompatible) toward mass spectrometry detection. Results of this study showed that a modified staining method using colloidal coomassie blue G-250 (CCB) is roughly similarly sensitive but lower protein spot detected compared with silver staining (SS) as indicated at the number of 303±26 and 693±14of protein resolved in both types of stained gels. The conventional methods of staining using commassie brilliant blue G-250 and R-250 only detected less number of protein spots(128±17and 78±11, respectively) compared to modified CCB staining method. As the commasie brilliant blue stain wasis known to be a very sensitive for mass spectrometry detection, the modified method of CCB was selected for further study on Puntius javanicus liver proteome.

Item Type: Article
Uncontrolled Keywords: P. javanicus,Proteomic, CBB R-250, CBB G-250, Silver nitrate
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: Munira
Date Deposited: 08 Mar 2018 03:10
Last Modified: 08 Mar 2018 03:10
URI: http://eprints.ums.edu.my/id/eprint/19118

Actions (login required)

View Item View Item