Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia

Jualang Azlan Gansau, and Goh, Keng Sean (2009) Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia. Borneo Science, 21. pp. 37-46. ISSN 1394-4339

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Abstract

Studies on eukaryotic signal transduction pathways have led to a variety of potential molecular targets for cancer therapy including Mitogen-activated Protein Kinase Kinase 1 (MKK1), Glycogen Synthase Kinase 3ß (GSK-3ß) and Type 1 Protein Phosphatase (PP1). The protein kinases and phosphatases in the signal transduction pathways play a vital role in mitogen activated protein (MAP) kinase signal transduction, tumorigenesis, apoptosis, and cancer metastasis. In this study, enetically engineered yeast strains were used as a model in the search for potential inhibitors against these signalling elements. Eleven species of medicinal plants were selected from various locations in Sabah, Malaysia, extracted and tested against MKK, GSK-3ß and PP1. Preliminary results showed the presence of potential MKK1 inhibitors in the crude extracts of Alphitonia excelsa, Cordyline terminalis and Tinospora crispa. However, no inhibitor was found against GSK-3ß and PP1. The crude extracts of Cordyline terminalis was further fractionated using a bioassay guided fractionation and four partially pure active fractions were isolated.

Item Type: Article
Uncontrolled Keywords: Inhibitor, Kinase, Phosphatase, Signal transduction, Medicinal plants
Subjects: Q Science > QR Microbiology
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Noraini
Date Deposited: 09 Mar 2018 07:16
Last Modified: 09 Mar 2018 07:16
URI: http://eprints.ums.edu.my/id/eprint/19240

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