Over-expression of the X-linked inhibitor of apoptosis protein (XIAP) delays serum deprivation-induced apoptosis in CHO-K1 cells

J., C. J. Liew and Tan, Wen Siang and B M Alitheen, Norjahan and Chan, Eng Seng and Tey, Beng Ti (2010) Over-expression of the X-linked inhibitor of apoptosis protein (XIAP) delays serum deprivation-induced apoptosis in CHO-K1 cells. Journal of Bioscience and Bioengineering, 110 (3). pp. 338-344. ISSN 1389-1723

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Official URL: http://dx.doi.org/10.1016/j.jbiosc.2010.02.017

Abstract

Serum deprivation inhibits cell growth and initiates apoptosis cell death in mammalian cell cultures. Since apoptosis is a genetically controlled cell death pathway, over-expression of anti-apoptotic proteins may provide a way to delay apoptosis. This study investigated the ability of the X-linked inhibitor of apoptosis protein (XIAP) to inhibit apoptosis induced by serum deprivation. Study includes evaluation of the ability of XIAP to prolong culture period and its effect on cell proliferation in serum-deprived media. The full length human XIAP was introduced into CHO-K1 cell lines and the effects of XIAP over-expression on the inhibition of apoptosis induced by serum-deprived conditions were examined. In batch cultures, cells over-expressing XIAP showed decreased levels of apoptosis and a higher number of viable cell under serum-deprived conditions compared to the control cell lines. The viability of control cells dropped to 40% after 2. days of serum deprivation, the XIAP expressing cells still maintained at a viability higher than 90%. Further investigation revealed that the caspase-3 activity of the CHO-K1 cell line was inhibited as a result of XIAP expression. © 2010 The Society for Biotechnology, Japan.

Item Type:Article
Uncontrolled Keywords:Apoptosis, CHO-K1, Flow cytometry, Mammalian cell culture; Serum deprivation, XIAP
Subjects:?? QH426-470 ??
Divisions:SCHOOL > School of Engineering and Information Technology
ID Code:1947
Deposited By:IR Admin
Deposited On:01 Mar 2011 15:57
Last Modified:17 Feb 2015 16:42

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