Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah

Nurul Ashira Azman, (2014) Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Helicobacter pylori is a pathogenic bacterium that has been associated with peptic ulcer disease and cancers of human gastrointestinal tract. Currently, c1arithromycin has remained as one of the most powerful antibiotics used as first line treatment for H. pylori infection. In Malaysia, 2.14% of c1arithromycin resistance H. pylori cases were reported. The small percentage indicates the low incidence of c1arithromycin resistance H. pylori in this country. This study aims to detect A2142G and A2143G mutations of 235 rRNA gene in H. pylori isolated from 5abah and to determine prevalence of c1arithromycin resistance in 5abah isolates using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). campylobacter-like organism (CLO) tested gastric biopsies were collected from Queen Elizabeth Hospital and subjected for DNA extraction. PCR amplification of 165 rRNA was performed to confirm the presence of H. pylori in the extracted DNA. Next, 235 rRNA gene was amplified and preceded for restriction fragment length polymorphism (RFLP) by restriction enzyme BsaI and MboII to study A2142G and A2143G mutations. From 67 gastric biopsies evaluated, only 41 were detected to be H. pylori positivity. Among 41 H. pylori positivity extracts, 29 isolates were successfully amplified at region 235 rRNA gene. Total two isolates has been detected with A2142G mutation and another one with A2143G mutation out of 25 isolates. Low-level of resistance was detected in Sabah isolates with only 4% of cases. This showed the uncommon occurrence in the local strain. This might due to the less usage of this antibiotic for H. pylori eradication treatment in this region. Therefore, clarithromycin can be continued as the first line drug to treat H. pylori strain. PCR-RFLP is one the reliable approaches in detecting mutations in H. pylori clarithromycin resistant strain

Item Type: Academic Exercise
Uncontrolled Keywords: mutation, Helicobacter pylori, DNA extraction, clarithromycin
Subjects: T Technology > TP Chemical technology
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 27 Mar 2018 07:25
Last Modified: 27 Mar 2018 07:25
URI: http://eprints.ums.edu.my/id/eprint/19585

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