Endotoxin removal and plasmid DNA recovery in various metal ion solutions

Clarence M. Ongkudon, and Michael K. Danquah, (2011) Endotoxin removal and plasmid DNA recovery in various metal ion solutions. Separation Science and Technology, 46 (8). pp. 1280-1282. ISSN 1520-5754

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Abstract

In this brief article, we report endotoxin precipitation using different types of metal ion in plasmid DNA purification. The work involved modification and improvement of the LAL chromogenic analysis to compensate for metal ion-LAL interaction. Results (Figs. 1 and 2) show that ZnSO4 gives an optimum combination of high endotoxin removal efficiency (∼91%) as well as high plasmid recovery (∼100%) compared to other metal ions tested.

Item Type: Article
Uncontrolled Keywords: free metal ion, lipopolysacharide, plasmid DNA, selective precipitation
Subjects: Q Science > QP Physiology
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Munira
Date Deposited: 27 Mar 2018 07:25
Last Modified: 27 Mar 2018 07:25
URI: http://eprints.ums.edu.my/id/eprint/19592

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