Evaluation of acetylcholinesterase source from fish, Tor tambroides for detection of carbamate

Siti Aqlima Ahmad, and Mohd Khalizan Sabullah, and Nor Aripin Shamaan, and Mohd Yunus Abd. Shukor, and Hussain Jirangon, and Ariff Khalid, and Mohd Arif Syed, (2016) Evaluation of acetylcholinesterase source from fish, Tor tambroides for detection of carbamate. Journal of Environmental Biology, 37. pp. 479-484. ISSN 2394-0379

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Abstract

Acetylcholinesterase (AChE) from the brain tissue of local freshwater fish, was isolated through affinity purification. Acetylthiocholine iodide (ATCi) was preferable synthetic substrate to purified AChE with highest maximal velocity (V ) and lowest biomolecular constant (K ) at 113.60 Umg and 0.0689 mM, respectively, with highest catalytic efficiency ratio (V /K ) of 1648.77. The optimum pH was 7.5 with sodium phosphate buffer as medium, while optimal temperature was in the range of 25 to 35 °C. Bendiocarp, carbofuran, carbaryl, methomyl and propoxur significantly lowered the AChE activity greater than 50%, and the IC value was estimated at inhibitor concentration of 0.0758, 0.0643, 0.0555, 0.0817 and 0.0538 ppm, respectively.

Item Type: Article
Uncontrolled Keywords: Acetylcholinesterase, Brain, carbamate, organophosphate, Tor Tambroides
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 28 Mar 2018 01:53
Last Modified: 28 Mar 2018 01:53
URI: http://eprints.ums.edu.my/id/eprint/19626

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