Strobilanthes crispus attenuates renal carcinogen, iron nitrilotriacetate (Fe-NTA)-mediated oxidative damage of lipids and DNA

Mohammad Iqbal, and Mahadimenakbar Mohd Dawood, and Chau, Ann Lie and Chin, Kim On (2010) Strobilanthes crispus attenuates renal carcinogen, iron nitrilotriacetate (Fe-NTA)-mediated oxidative damage of lipids and DNA. Molecular and Cellular Biochemistry, 341 (1-2). pp. 271-277. ISSN 0300-8177

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Official URL: http://dx.doi.org/10.1007/s11010-010-0458-x

Abstract

This study was aimed to evaluate the effect of Strobilanthes crispus extract for possible protection against lipid peroxidation and DNA damage induced by iron nitrilotriacetate (Fe-NTA) and hydrogen peroxide (H 2O2). Fe-NTA is a potent nephrotoxic agent and induces acute and subacute renal proximal tubular necrosis by catalyzing the decomposition of H2O2-derived production of hydroxyl radicals, which are known to cause lipid peroxidation and DNA damage. Incubation of postmitochondrial supernatant and/or calf thymus DNA with H 2O2 (40 mM) in the presence of Fe-NTA (0.1 mM) induces lipid peroxidation and DNA damage to about 2.3-fold and 2.9-fold, respectively, as compared to control (P < 0.05). In lipid peroxidation protection studies, S. crispus treatment showed a dose-dependent inhibition (45-53% inhibition, P < 0.05) of Fe-NTA and H2O2 induced lipid peroxidation. Similarly, in DNA damage protection studies, S. crispus treatment also showed a dose-dependent inhibition (18-30% inhibition, P < 0.05) of DNA damage. In addition, the protection was closely related to the content of phenolic compounds as evident by S. crispus extract showing the value of 124.48 mg/g total phenolics expressed as gallic acid equivalent (GAE, mg/g of extract). From these studies, it is concluded that S. crispus inhibits peroxidation of membrane lipids and DNA damage induced by Fe-NTA and H2O2 and possesses the potential to be used to treat or prevent degenerative diseases where oxidative stress is implicated. © 2010 Springer Science+Business Media, LLC.

Item Type:Article
Uncontrolled Keywords:DNA damage, Hydrogen peroxide, Iron nitrilotriacetate, Lipid peroxidation, Oxidative stress, Strobilanthes crispus
Subjects:?? TP248.13-248.65 ??
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:1968
Deposited By:IR Admin
Deposited On:02 Mar 2011 12:33
Last Modified:17 Feb 2015 17:08

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