Tetraplex PCR assay involving double gene-sites discriminates beef and buffalo in Malaysian meat curry and burger products

M.A. Motalib Hossain, and Md. Eaqub Ali, and Sharifah Bee Abd Hamid, and S.M. Azad Hossain, and Asing, and Nina Naquiah Ahmad Nizar, and Mohammad Nasir Uddin, and Lokman Ali, and Md. Asaduzzaman, and Md. Jahurul Haque Akanda e, (2017) Tetraplex PCR assay involving double gene-sites discriminates beef and buffalo in Malaysian meat curry and burger products. Food Chemistry, 224. pp. 97-104. ISSN 0308-8146

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Abstract

Replacement of beef by buffalo and vice versa is frequent in global markets, but their authentication is challenging in processed foods due to the fragmentation of most biomarkers including DNA. The shortening of target sequences through use of two target sites might ameliorate assay reliability because it is highly unlikely that both targets will be lost during food processing. For the first time, we report a tetraplex polymerase chain reaction (PCR) assay targeting two different DNA regions in beef (106 and 120-bp) and buffalo (90 and 138-bp) mitochondrial genes to discriminate beef and buffalo in processed foods. All targets were stable under boiling, autoclaving and microwave cooking conditions. A survey in Malaysian markets revealed 71% beef curries contained buffalo but there was no buffalo in beef burgers. The assay detected down to 0.01 ng DNA and 1% meat in admixed and burger products.

Item Type: Article
Uncontrolled Keywords: Double gene targets , Biomarkers , Target stability
Divisions: FACULTY > Faculty of Food Science and Nutrition
Depositing User: Noraini
Date Deposited: 21 Jun 2018 05:57
Last Modified: 21 Jun 2018 05:57
URI: http://eprints.ums.edu.my/id/eprint/20329

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