Genotyping of XRCC1 polymorphisms in breast cancer

Nalini Krishnan, (2008) Genotyping of XRCC1 polymorphisms in breast cancer. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Breast cancer is most common cancer among women in Malaysia. The second report of the Malaysian National Cancer Registry in 2004 alluded that 46.2 in 100,000 populations diagnosed with breast cancer in 2003. Breast cancer is more common amongst Chinese and Indian women compared to Malay women in Malaysia. Although the incidence is low, breast cancer in Malaysia could be considered as the leading cause of cancer death among women. This study investigated the genetic polymorphisms of DNA repair gene X-ray repair cross complementing factor 1 (XRCCI) at codon 194 (Arg to Trp), may increase the susceptibility to breast cancer by modifying individual DNA repair capability. Arg194Trp polymorphism at the XRCCI gene were analyzed by using Restriction Fragment Length Polymorphisms (RFLP) assay based on DNA samples of five patients with breast cancer and one healthy subject. Polymerase Chain Reaction (PCR) was carried out by using specific primers to amplify the region XRCCI containing Argl94Trp site. The PCR product generated a single band at 485 bp as expected. Then, the PCR product digested with restriction enzyme PvuII and the products were analyzed with 2% agarose gel electrophoresis. The frequency of the wild type Arg/Arg genotype was more pronounced among cases (80%) compared with heterozygote variant Arg/Trp (20%). No subjects carried the codon 194-Trp/Trp (0%). There is no polymorphism detected in control. In conclusion, based on this limited data, XRCC1 Arg194Trp does not contribute to breast cancer in Sabah bumiputera population. However, for this type of analysis a very large sample size is essential to get an accurate result

Item Type: Academic Exercise
Uncontrolled Keywords: DNA repair, breast cancer, genetic polymorphisms, Polymerase Chain Reaction
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: Munira
Date Deposited: 19 Jul 2018 06:45
Last Modified: 19 Jul 2018 06:45
URI: http://eprints.ums.edu.my/id/eprint/20536

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