In vitro germination and plantlet establishment ofLabisia pumila (Bl.) F. Vill.

Hartinie Marbawi, and G. Azlan Jualang, (2007) In vitro germination and plantlet establishment ofLabisia pumila (Bl.) F. Vill. Scientia Horticulturae, 115 (1). pp. 91-97. ISSN 0304-4238

[img] Text
In vitro germination and plantlet establishment of Labisia pumila (Bl.) F. Vill..pdf

Download (52kB)

Abstract

In vitro seeds germination and plantlet establishment of Labisia pumila were studied in this report. The seeds obtained from the mature fruits of L. pumila were sterilized and cultured on Murashige and Skoog (MS) solid media supplemented with 1–3 μM of 6-benzylaminopurine (BAP) and 3% (w/v) sucrose. The presence of BAP in the medium significantly affects seeds germination. High percentage of seeds germination (up to 90%) was successfully achieved after 2 weeks of culture on medium supplemented with 2 μM BAP. Up to 70% of explants produced shoots through direct regeneration from newly emerged epicotyls after 5 weeks of culture. The average of 8.1 ± 1.0 shoots per explant obtained on media treated with 2 μM BAP. Seedlings were further transferred to growth media fortified with different types of cytokinin. Result observed after 12 weeks showed that medium supplemented with 1 μM zeatin (ZEA) promote the highest growth with an average of 2.9 ± 1.0 cm shoot length and 7.7 ± 3.2 leaves per explant after 12 weeks. In addition, medium added with 2 μM BAP and supplemented with 3–4% (w/v) of sucrose promote the best growth i.e., 3.0 ± 0.6 shoots per explant, 2.27 ± 0.2 cm length and 4.3 ± 0.5 leaves per explant.

Item Type: Article
Uncontrolled Keywords: Medicinal plant, Micropropagation, Myrsinaceae, Plant growth regulators
Subjects: Q Science > QK Botany
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 05 Mar 2019 04:28
Last Modified: 05 Mar 2019 04:28
URI: http://eprints.ums.edu.my/id/eprint/21486

Actions (login required)

View Item View Item