Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens

Kalavathy Ramasamy, and Norhani A. P. Abdullah, and Wong, Clemente Michael Vui Ling and Chinna Karuthan, and Yin, Wan Ho (2010) Bile salt deconjugation and cholesterol removal from media by Lactobacillus strains used as probiotics in chickens. Journal of the Science of Food and Agriculture, 30 (1). pp. 65-69. ISSN 0022-5142

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Official URL: http://dx.doi.org/10.1002/jsfa.3780

Abstract

BACKGROUND: Bile salt deconjugation by Lactobacillus strains is often closely linked to bile tolerance and survival of the strains in the gut and lowering of cholesterol in the host. The present study investigated the deconjugation of bile salts and removal of cholesterol by 12 Lactobacillus strains in vitro. The 12 strains were previously isolated from the gastrointestineal tract of chickens. RESULTS: The 12 Lactobacillus strains could deconjugate sodium glycocholate (GCA, 16.87-100%) and sodium taurocholate (TCA, 1.69-57.43%) bile salts to varying degrees, with all strains except L. salivarius I 24 having a higher affinity for GCA. The 12 Lactobacillus strains also showed significant (P < 0.05) differences in their ability to remove cholesterol from the growth medium (26.74-85.41%). Significant (P < 0.05) correlations were observed between cholesterol removal and deconjugation of TCA (r = 0.83) among the L. reuteri strains (C1, C10 and C16) and between cholesterol removal and deconjugation of TCA (r = 0.38) and GCA (r = 0.70) among the L. brevis strains (I 12, I 23, I 25, I 211 and I 218). In contrast, although L. gallinarum I 16 and I 26 and L. panis C 17 showed high deconjugating activity, there was no correlation between cholesterol removal and deconjugation of bile salts in these strains. CONCLUSION: The results showed that the 12 Lactobacillus strains were able to deconjugate bile salts and remove cholesterol in vitro, but not all strains with high deconjugating activity removed cholesterol effectively. © 2009 Society of Chemical Industry.

Item Type:Article
Uncontrolled Keywords:Bile salt, BSH, Cholesterol, Deconjugation, Lactobacillus, Probiotics
Subjects:?? TP248.13-248.65 ??
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:2203
Deposited By:IR Admin
Deposited On:11 Mar 2011 12:12
Last Modified:18 Feb 2015 10:25

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