Genetic analysis of the resistance to rice blast in the BC2F1 population derived from MR263 × Pongsu Seribu 1

M. M. Hasan, and M. Y. Rafii, and M. R. Ismail, and M. Mahmood, and H. A. Rahime, and M. A. Latif, and Md. Amirul Alam, and Fahim Ahmad, and M. A. Malek, (2018) Genetic analysis of the resistance to rice blast in the BC2F1 population derived from MR263 × Pongsu Seribu 1. Biotechnology & Biotechnological Equipment, 32 (5). pp. 1134-1140. ISSN 1310-2818

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Abstract

Rice blast disease, caused by Magnaporthe oryzae, is the most important and most devastating rice disease globally. For genetic analysis of resistance to rice blast, the present study used a BC2F1 population developed through marker-assisted backcrossing from a cross between blast susceptible MR263 and blast resistant Pongsu Seribu 1 (PS1). We selected out of 450 markers, 65 polymorphic simple sequence repeat markers, including the Pi gene-based markers, and identified 16 markers associated with blast resistance that showed heterozygous bands in theBC2F1 population. Of the 16 polymorphic markers, only eight (RM5961, RM263, RM163, RM224, RM262, RM168, RM229 and RM169) showed a good fit to the expected segregation genotypic ratio (1:1) for the single dominance gene model (df¼1.0, P<0.05), according to chi-square (v2)analysis. An analysis of phenotypic data of the BC2F1 population also showed a good fit to the expected phenotypic ratio (1:1; R:S) for resistant and susceptible plants. The resistance to blast pathotype P7.2 in PS1 is most likely controlled by a single dominant gene that is linked to the eight markers we identified. These markers could be used in marker-assisted selection programmesto develop a durable blast resistant rice variety.

Item Type: Article
Uncontrolled Keywords: genetic analysis, blast resistant, Magnaporthe oryzae, MABC, Pongsu Serebu 1 and MR263
Subjects: S Agriculture > SB Plant culture
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 12 Jul 2019 07:21
Last Modified: 12 Jul 2019 07:21
URI: http://eprints.ums.edu.my/id/eprint/22628

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