Hepatotoxic and Gonadotoxic effects of low dose of Insecticide Diazinon in male rats

Urban John Arnold D'Souzaa, and Zainal Arifin Mustapha, and Chong, Thau Leong (2012) Hepatotoxic and Gonadotoxic effects of low dose of Insecticide Diazinon in male rats. (Unpublished)

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OVer the decades, more than hundred thousands of synthetic pestlcldes have been invented as a helpful source to eradicate nuisance pests and to maintain adequate food supply. Diazinon [O,o-c.tiethyl-O-(2 -isopropyl-6-methyl-pyrimidine-4-yl) phosphoroth iote], an organophosphate insecticide has been widely used in agriculture and domestic scale. It is rapidly degraded by microbes in the environment and to a small extent by exposure to sun light. However, its ubiquitous and negligible amount in the environment presents a continuing health hazard to exposed farmers. Human beings can be potentially exposed to this semi-volatile insecticide through inhalation, ingestion and dermal contacts. Diazinon toxicity was propossj via oxidative stress by lipid peroxidation and relatiVely was less studied in male reproductive system. Hence the current work aimed to investigate the dose dependant adverse effects of diazinon on few physiological, biochemical and histopathological changes in liver and testis of adult male SpragueDawley rats based on LDso as a measure of the lowest possible exposure in agricultural environment The rats were gavaged with diazinon at 0.0, 0.2, 0.3 and 0.6% of LDso (>5000 mg/kg body weight) in 3 durations of 1, 2 and 8 weekes). Activity of liver aspartate aminotransferase, alkaline aminotransferase, alkaline phosphatase, and lipid peroxidation exhibited an increase with consensus inhibition of reduced glutathione and catalase levels. Parallel with the biochemical changes, diazinon treatment Significantly enhanced the damage in testis. The levels d lipid peroxidation was significantly increased with 0.06% dose in 1 week treatment and all doses in 2 and 8 weeks treatment, following with the signifICant decrement in reduced glutathione levels in all doses of 2 and 8 week and catalase diminishing trend. Diazinon Significantly decreased serum testosterone levels in 8 week treatment and was accompanied with an increased incidence of sperm abnormality in testis. Increase in serum lactate dehydrogenase activity with a qualitative derangement in liver and testis histology possibly induces cytotoxic effects. The toxicity of diazinon with low dose exposure study revealed a dose and time dependant response in the parameters of this study. Different antioxidants and enzymes showed signifICant alterations with an increase in lipid peroxidation. Diazinon at low doses is cytotoxic to liver and germ cell lines.

Item Type: Research Report
Uncontrolled Keywords: Diazinon , serum lactate dehydrogenase , lipid peroxidation
Subjects: R Medicine > R Medicine (General)
Divisions: SCHOOL > School of Medicine
Depositing User: Noraini
Date Deposited: 17 Jul 2019 10:04
Last Modified: 18 Jul 2019 13:46
URI: http://eprints.ums.edu.my/id/eprint/22769

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