In vitro propagation and Pharmaceutical evaluation of Kappaphycusalvareziiand Eucheuma Denticulatum, two Commercially important seaweeds of Sabah

Yong, Wilson Thau Lym (2012) In vitro propagation and Pharmaceutical evaluation of Kappaphycusalvareziiand Eucheuma Denticulatum, two Commercially important seaweeds of Sabah. (Unpublished)

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Abstract

In Sabah, the problems associated with using conventional methods to cultivate seaweeds are mainly suitable sites for farming, seasonal variations and disease infestations. In vitro tissue culture techniques can solve the problems currently faced by the local seaweed industry and facilitate the propagation of commercially important genotypes. This research was carried out to optimize the suitable conditions for the micropropagation of Kappaphycus alverazii. Culture media, explants size, aeration activity and light illuminescence were found to be the critical factors that affect the growth of Kappaphycus alverazii. The optimum conditions for culturing seaweeds were formed to be initial explants of 0.5 to 1.0 %w/v, 50 % PES media enrichment and supplemented with 2.5 g/I BAP and 1.0 g/I IAA, light intensity of 6,000 lux, continuous aeration and temperature of 24 to 27°C. In order to achieve mass cultivation of seedlings, a customized airlift photobioreactor was constructed to provide the optimal culture conditions to the seaweed cultivation. The daily growth rate of cultures in the photobioreactor (6.0 %/day) was found to be higher than the growth rate obtained from the culturing flask (4.26 %/day) and the cultivation in sea (5.5 %/day). A study involving the profiling of protein expression between sea cultivated seaweed and clonally propagated seaweed revealed that short peptides with a molecular mass of 5-10 kDA were produced in significant amounts in naturally cultivated seaweeds but the production was stopped after first 12-week of laboratory culture. These peptides were revealed to be in the class of Lectin by MALDI-TOF method and the reappearance of these peptides after 12 weeks of cultivation may indicate that the propagules are ready to be transferred to the natural environment as they possess defence systems for adaptation to the wild. Thus, Lectins can be applied as a biomarker to monitor the quality of seaweed cultures in vitro and this biomarker can be highly beneficial to the seaweed farming industry. BeSides, review of the current literatures also indicated that natural seaweeds are an excellent source of bioactive compounds which possess antioxidant, antimicrobial and antitumor activities. In the present study, 90% methanolic, 70% acetonic and aqueous extracts from Kappaphycus a/varezii (strain Crocodile, Giant and Brown) and Kappaphycus striatum were used to inhibit the growth of HeLa cell lines. MTS assay was carried out to determine the proliferation of HeLa cells in the presence of various seaweed extracts. Results indicated that the most effective extract to inhibit cell growth was found to be 500 ug/ml of aqueous extract of K. striatum followed by 500 ug/ml of methanolic extract of same species, with 53.52% and 43.71% of growth inhibition, respectively. Most of the methanolic extracts from Kappaphycus seaweeds have shown positive growth inhibition to the HeLa cell lines as compared to acetonic and aqueous extracts. Further studies are suggested to identify and purify the specific anti-tumoral compounds for the development of cancer therapy.

Item Type: Research Report
Uncontrolled Keywords: Seaweeds , micropropagation , Kappaphycus alverazii , HeLa cell
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Noraini
Date Deposited: 18 Jul 2019 13:50
Last Modified: 18 Jul 2019 13:50
URI: http://eprints.ums.edu.my/id/eprint/22815

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