Detection of water stress-induced Gene In Maize under drought stress condition

Lum, Mok Sam Detection of water stress-induced Gene In Maize under drought stress condition. (Unpublished)

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Abstract

Plant growth and productivity are greatly affected by water stress. Maize is an important cash crop In Malaysia where It often suffers from drought stress. This study focused on determines the water stress-Induced gene In maize under drought stress condition In Sabah. The young maize leaves which at the weIght range of 100 :t 5 mg were collected for totiJl RNA isolation process and the RNA yield range Is 50- 200 Jig. There were undetectiJble amplified band in PCR process for Samples 1 and 2. While, a specific band located approximate 585 bp was amplified by usIng AHLEA primer set for Samples ~ 4 and 5. Sample 5 shown most clearance of amplified band which was further underwent pUrification process. This band was eluted and cloned Into pGE~ -T Easy Vector. There were 10 white colonies and 11 light blue colonies were formed on LB plate supplemented with Amplcillin/ X-Gall IPTG. After mlniprep Isolation, these plasmids were digested wIth EcoRI. Two different bands which located at 400 bp and 230 bp were formed by EcoRL These result showed that there Is stress-Induced gene In maize under drought stress condition.

Item Type: Research Report
Uncontrolled Keywords: Plant , water stress , Malaysia , EcoRI
Subjects: S Agriculture > SB Plant culture
Depositing User: Noraini
Date Deposited: 29 Jul 2019 08:23
Last Modified: 29 Jul 2019 08:23
URI: http://eprints.ums.edu.my/id/eprint/23160

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