Comparison of Glycerol and Ethylene Glycol in old coconut water for short term preservation of native rooster semen

Ooi, Phaik Sim Comparison of Glycerol and Ethylene Glycol in old coconut water for short term preservation of native rooster semen. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The objective of this study was to determine the effect of using different concentrations of glycerol (G) and ethylene glycol (EG) respectively in old coconut water (OCW) for short term preservation of native rooster semen under chilled temperature (5° C). This study was carried out at Faculty of Sustainable Agriculture (FSA), Universiti Malaysia Sabah (UMS) where the rooster semen collection was conducted at poultry house while the semen analysis was conducted in Anatomy and Physiology Laboratory. The duration of this study was from August until October 2016. In this study, three to four native roosters were randomly selected for semen collection each time where the fresh semen collected were pooled together in an appendoft tube. It was repeated once a week for three replications by the adoption of Completely Randomized Design (CRD) in this study. The fresh pooled semen was then evaluated based on the characteristics of semen volume, concentration, colour and consistency, mass motility (MMOT) and individual forward motility. In this study, the mean volume of fresh pooled semen obtained was 0.17±0.01 ml with the mean colour and consistency scored at 4.33±0.58 and mean concentration of 1.06±0.03x109 sperm/ ml. Moreover, the MMOT obtained was 5.67±0.58 with the individual forward motility of 90.00±0.00%. The fresh pooled semen with minimum 85% of individual forward motility was selected to proceed the further experimental treatments where 20 µL of fresh pooled semen was mixed with 680 µL of each of the respective treatment group. The treatment groups were composed of control (OCW diluent without any cryoprotectant), G and EG with the concentrations of 2%, 4% and 6% respectively in OCW diluent. The seven appendoft tubes containing treated semen were stored in refrigerator under chilled temperature of S°C over 72 hour intervals. Evaluation was done based on the aspects of percentages of individual forward motility, live sperm count and total sperm abnormalities which included the percentage of head, midpiece and tail abnormalities. In this study, EG showed the best outcomes with the concentrations of 4% and 6% as compared to other treatment groups over the 72 hour intervals where both 4% and 6% of EG were significantly different with others in term of individual forward motility and live sperm count at 36th hour onwards. At 72th hour of observation, the individual forward motility for the 4% and 6% of EG were 26.58±1.24% and 24.62±2.12% respectively meanwhile their live sperm count was 26.67±0.00% and 25.56±1.11 % respectively. However for the aspects of sperm abnormalities, there were no significant differences found between the treatment groups over the 72th hour intervals. Apart from this, it was also found that the inclusion of EG and Gas cryoprotectants in semen diluent gave better cryoprotective effects for the native rooster spermatozoa under chilled temperature of 5°C as compared to the control treatment (without any cryoprotectant) which was found to be deteriorated rapidly in this study. Thus, it can be concluded that cryoprotectant is important for semen preservation under chilled temperature. Both 4% and 6% of EG can be interacted well with OCW diluent for the short term preservation of native rooster semen under chilled temperature for about 72 hour intervals.

Item Type: Academic Exercise
Uncontrolled Keywords: Old coconut water diluent , Ethylene Glycol , Glycerol, native rooster semen
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Noraini
Date Deposited: 31 Oct 2019 07:47
Last Modified: 31 Oct 2019 07:47
URI: http://eprints.ums.edu.my/id/eprint/23928

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