In vitro maturation of Porcine Oocytes

Tan, Rebecca Ai Sim (2018) In vitro maturation of Porcine Oocytes. Universiti Malaysia Sabah. (Unpublished)

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An experiment was conducted in the Plant Physiology Laboratory at the Faculty of Sustainable Agriculture, Universiti Malaysia Sabah throughout June until July 2017. The purpose of the experiment was to examine the effects of bovine serum albumin (BSA) and polyvinyl alcohol (PVA) on the cumulus expansion and nuclear maturation of porcine oocytes. Porcine ovaries were collected from the Sandakan Municipal Council pig slaughterhouse in Mile 8, suitable cumulus-oocyte-complexes (COCs) were aspirated from ovaries with corpus luteum present (CL+) and subsequently matured in tissue culture media 199 (TCM-199) containing either 5% BSA or 4% PVA. The COCs were then incubated at 37.5° C at 20% oxygen (02) and 5% carbon dioxide (CO2) for 48 hours. The cumulus expansion was observed at 24 and 48 hours under an inverted light microscope whilst the nuclear maturation was observed after 48 hours once the oocytes were mechanically denuded, stained with aceto-orcein stain and viewed under an inverted light microscope at 100x magnification. From this experiment, we have found that oocytes matured in BSA (10.8% and 37.8%) had more oocytes expanding compared to PVA (2.2% and 2.2%) for cumulus expansion at 24 hours and 48 hours respectively. The nuclear maturation of oocytes in BSA (43.2%) at 48 hours that had achieved germinal vesicle break down (GVBD) was also significantly higher than PVA (3.7%) however, both mediums did not support nuclear maturation past GVBD. This concludes that BSA has a better potential for in vitro maturation usage in porcine oocytes as compared to PVA.

Item Type: Academic Exercise
Uncontrolled Keywords: Agriculture , Bovine serum albumin (BSA), Porcine
Subjects: S Agriculture > S Agriculture (General)
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Noraini
Date Deposited: 12 Nov 2019 00:18
Last Modified: 12 Nov 2019 00:18

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