The effects of stingless bee honey in tris extender on semen quality of Boer Goat

Sebastian Abin, (2018) The effects of stingless bee honey in tris extender on semen quality of Boer Goat. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The objective of this research was to determine the effects of stingless bee honey in Tris extender on semen quality of Boer goat. The research were conducted at Faculty of Sustainable Agriculture (FSA), Universiti Malaysia Sabah (UMS). Collection of Boer goat semen were done at the Goat Rearing Facility while semen evaluation were done at the Anatomy and Physiology Laboratory. Three (3) male Boer goat were randomly selected for this research and semen collection were done once a week using an Artificial Vagina (AV). Semen were collected 3 times and the experiment adopted the Completely Randomized Design (CRD). The fresh Boer goat semen were assessed in term of volume, colour, mass motility, and concentration. For this research, the collected Boer goat semen have a mean volume of 1.83±0.17 ml, concentration of 2.21±0.13x109 sperm/ml, a creamy white appearance, and a mean mass motility of 5.00. Fresh semen with a minimum of 80% individual progressive motility were selected and proceed to further experimental treatment. Four ( 4) treatments is conducted on the semen, in which the first treatment (Tl) serve as the control containing only Tris Buffer Solution {TBS) as the semen extender, while the second {T2), third {TI), and fourth {T4) treatment group contains TBS + 0.5%, 1.0%, and 1.5% volume per volume (v/v) of stingless bee honey respectively. Semen sample from each treatment were then assessed in term of sperm individual progressive motility, sperm viability and sperm abnormality through microscopic observation and eosin-nigrosin staining procedure. From this research, TBS + 0.5% stingless bee honey produced the best result together with the control treatment in term of sperm motility and viability. For sperm abnormality parameters, sperm from Tl, T2, TI, and T4 showed no significant difference from each other. Therefore, it can be concluded that addition of 0.5% stingless bee honey in Tris extender are comparable to the control treatment, and can preserve sperm motility and viability up to 24 hours.

Item Type: Academic Exercise
Uncontrolled Keywords: Boer goat , bee honey
Subjects: S Agriculture > SF Animal culture
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Noraini
Date Deposited: 12 Nov 2019 00:18
Last Modified: 12 Nov 2019 00:18
URI: http://eprints.ums.edu.my/id/eprint/24064

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